| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174699 |
| Chromosome: | chromosome 7 |
| Location: | 1752330 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325718 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCACCGGCGTCAGCATCAGCGGCATGGT |
| Internal bar code: | ACTAGGTAAGGGAAATCGACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 944 |
| LEAP-Seq percent confirming: | 63.2205 |
| LEAP-Seq n confirming: | 9521 |
| LEAP-Seq n nonconfirming: | 5539 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAATGGCACTTACGGTGG |
| Suggested primer 2: | AGCCCCTGGTCCTTAGTCAT |