| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174707 |
| Chromosome: | chromosome 13 |
| Location: | 3618127 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588501 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCGACAGGCCCTTCGCCTTGATGGCC |
| Internal bar code: | GCTGAGACTGGACTATACCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 790 |
| LEAP-Seq percent confirming: | 89.5474 |
| LEAP-Seq n confirming: | 1662 |
| LEAP-Seq n nonconfirming: | 194 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCAGAACAGCATCACAA |
| Suggested primer 2: | GGGGCAACTCACAAACATCT |