| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174719 |
| Chromosome: | chromosome 7 |
| Location: | 4501527 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343500 | (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCTAAGCCGTGCCTCCCAGCCTGGCAT |
| Internal bar code: | CGAACGTTCGACCGTTGCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 998 |
| LEAP-Seq percent confirming: | 85.1818 |
| LEAP-Seq n confirming: | 3725 |
| LEAP-Seq n nonconfirming: | 648 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCTCCGCATACAATCCC |
| Suggested primer 2: | ACTGCGACCAGGTAATCCAC |