Insertion junction: LMJ.RY0402.174722_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.174722
Chromosome:chromosome 1
Location:2449089
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g013700 ASC1,VDAC1 (1 of 2) K15040 - voltage-dependent anion channel protein 2 (VDAC2); Voltage-dependent anion-selective channel protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGTGGTGGTGGTTTGTCGTAGTAGCGGG
Internal bar code:GGGGAGCTACTCTCAGCAGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1011
LEAP-Seq percent confirming:99.5462
LEAP-Seq n confirming:14478
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGGCTTCTGAGAGTCCTG
Suggested primer 2:CCAGCCCAAACTAAACCAAA