| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174731 |
| Chromosome: | chromosome 12 |
| Location: | 4580521 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522450 | SEC31 | (1 of 1) K14005 - protein transport protein SEC31 (SEC31); COP-II coat subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCCTAAGGCTTCACCGCAGCTTGGTGG |
| Internal bar code: | ATTGCCCTGAAACCGGTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1291 |
| LEAP-Seq percent confirming: | 97.4359 |
| LEAP-Seq n confirming: | 266 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTCGGGAAGCTATCCGTG |
| Suggested primer 2: | ATACAACCAGTACCAGCCGC |