| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174741 |
| Chromosome: | chromosome 11 |
| Location: | 1639151 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467770 | PGK1 | Phosphoglycerate kinase, chloroplastic; (1 of 1) 2.7.2.3 - Phosphoglycerate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGCAATTTACTTCTCGTCCAGGGCGGC |
| Internal bar code: | AACTGAGATGGGTCCGGAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 99.5498 |
| LEAP-Seq n confirming: | 1548 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAACACTGCAATGCGAAA |
| Suggested primer 2: | CAAGAAGCTGGAGGAGATGG |