| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174742 |
| Chromosome: | chromosome 13 |
| Location: | 5128355 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGCCCGGCCGCTCAGCCACGCTTTGAC |
| Internal bar code: | GGGTCACTAGGGACGCATGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 93.7182 |
| LEAP-Seq n confirming: | 2760 |
| LEAP-Seq n nonconfirming: | 185 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCAATGAAGATGCGGGAT |
| Suggested primer 2: | GTGACGGAGTAGAGGTTGCC |