| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174769 |
| Chromosome: | chromosome 10 |
| Location: | 1312783 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427550 | (1 of 3) IPR022751//IPR029044 - Alpha-mannosyltransferase // Nucleotide-diphospho-sugar transferases | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCACCGTGACTGCCACGGGACCACGCCA |
| Internal bar code: | AAGCGTTTTGAGTACAGAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1107 |
| LEAP-Seq percent confirming: | 99.647 |
| LEAP-Seq n confirming: | 2258 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGTAAACTGTCGTGGTG |
| Suggested primer 2: | GAGCGACACCTTCAAAAAGC |