| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174777 |
| Chromosome: | chromosome_12 |
| Location: | 3732135 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g514850 | HSP90C | Heat shock protein 90C | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGACCATGCCGCTGCCTTCGTGATCTCGT |
| Internal bar code: | GACAGATTCGGCGACTTGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 98.7921 |
| LEAP-Seq n confirming: | 1145 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATTCCACAACCACAACT |
| Suggested primer 2: | CCTTATGGCGAGAGCTTCTG |