| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174785 |
| Chromosome: | chromosome_17 |
| Location: | 5462180 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g737200 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGAGGCTGTGGTCGAGCTTTGAGGGGTACG |
| Internal bar code: | CCGGGCGTCAGCGGTGGTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 430 |
| LEAP-Seq percent confirming: | 98.8506 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTATGGTCAGGGCGTGT |
| Suggested primer 2: | GTAATGGGAGCACAGTCCGT |