| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174786 |
| Chromosome: | chromosome 6 |
| Location: | 3720542 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278148 | (1 of 2) K18121 - glyoxylate/succinic semialdehyde reductase (GLYR) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTCTCGCCACGGCCGGCTTCAGGTGACG |
| Internal bar code: | CGGCGGATTACTCCAATCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 97.5132 |
| LEAP-Seq n confirming: | 1294 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAATGGGGGTTACTTCAT |
| Suggested primer 2: | GTACTTGCTAGGCAGGCGAC |