| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174788 |
| Chromosome: | chromosome 5 |
| Location: | 3228953 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239950 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTAGCGGCCCTCTCCCTCCCGGTGCAT |
| Internal bar code: | GAAGCCGGGCATAGTGTGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1017 |
| LEAP-Seq percent confirming: | 99.7279 |
| LEAP-Seq n confirming: | 733 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAACGTCAGTCCCCATGC |
| Suggested primer 2: | TACCGTGTGTGTGGTGTGTG |