| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174797 |
| Chromosome: | chromosome 12 |
| Location: | 9550600 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546027 | (1 of 2) PF13472 - GDSL-like Lipase/Acylhydrolase family (Lipase_GDSL_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGCGCGGCCTGCGCAGCCCAGAAGCTT |
| Internal bar code: | CGGGCCTGTAGGCTCAAGCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 15 |
| LEAP-Seq percent confirming: | 89.2857 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACCGCCAGCGCTACTACT |
| Suggested primer 2: | GGCAAGCAGTGATCTTGTCA |