| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174811 |
| Chromosome: | chromosome 2 |
| Location: | 2544338 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092600 | CPX2 | Coproporphyrinogen III oxidase; (1 of 1) PTHR10755//PTHR10755:SF3 - COPROPORPHYRINOGEN III OXIDASE, MITOCHONDRIAL // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGGGATTGTGTGCTCCAGCCAACACCC |
| Internal bar code: | CTATTCACCCTTACTTGAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 973 |
| LEAP-Seq percent confirming: | 99.48 |
| LEAP-Seq n confirming: | 3252 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTGCATAGGCCAACAAC |
| Suggested primer 2: | ATTAACACGCATTCGGCTTC |