Insertion junction: LMJ.RY0402.174811_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.174811
Chromosome:chromosome 5
Location:1202067
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g245350 (1 of 1) IPR003582//IPR009011 - ShKT domain // Mannose-6-phosphate receptor binding domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCTCGTGCCCAGCTTCGCTGCCCCCGTC
Internal bar code:TCTAAGGGACAGGCTTATCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:90
LEAP-Seq percent confirming:99.3548
LEAP-Seq n confirming:154
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCTACGCCTTCACTTCTT
Suggested primer 2:AAATTGCGTGAAACCTACGG