| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174813 |
| Chromosome: | chromosome 3 |
| Location: | 9004263 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g209841 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGGGTTGGGGGTGGGAGGCGTGGGCGG |
| Internal bar code: | TGGTAAATCCTCACAAGCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.3466 |
| LEAP-Seq n confirming: | 2889 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATGGTGACGTGTGTTGT |
| Suggested primer 2: | CACTCTCCAGCATAGGGAGC |