| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174817 |
| Chromosome: | chromosome 7 |
| Location: | 5028898 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347250 | FIP37 | Target of FKB12/rapamycin complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGCTGGATTAGCTCGGGTTCCATAGC |
| Internal bar code: | CACAGGAGATGGGGGTAGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 98.7574 |
| LEAP-Seq n confirming: | 1510 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTTGGTGGGTGTTGTGTG |
| Suggested primer 2: | CGTACAGGCGTGTAGAAGCA |