| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174836 |
| Chromosome: | chromosome 12 |
| Location: | 6335543 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537350 | NZP1 | Zygote-specific hydroxyproline-rich glycoprotein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGGCCTCTGTGCCCGTGTTCTGCTGTG |
| Internal bar code: | TCGCTCGACCTGGCCACCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 99.6582 |
| LEAP-Seq n confirming: | 11953 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | COULD_NOT_FIND_PRIMER |
| Suggested primer 2: | TTGGAAGTTTTCTTGTGGGG |