| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174838 |
| Chromosome: | chromosome 3 |
| Location: | 6613802 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196550 | Found in Chlamydomonas, Volvox and Micromonas but not strongly conserved elsewhere; (1 of 5) PF03992 - Antibiotic biosynthesis monooxygenase (ABM) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCCGAGAAGCTGAGCTGGTAGCTCAGG |
| Internal bar code: | GGCCTCGCCACGAGCGTCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 99.2849 |
| LEAP-Seq n confirming: | 833 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCGCGTCCTTAAGCTAT |
| Suggested primer 2: | CGAGCTCACTTTGCCATGTA |