Insertion junction: LMJ.RY0402.174839_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.174839
Chromosome:chromosome 9
Location:6086785
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g403330 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTAGGCAGGGGGTTTGGCGATCGGGCGA
Internal bar code:CTGACTGTGCTGGGGTTCGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:922
LEAP-Seq percent confirming:73.8095
LEAP-Seq n confirming:155
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGGATGAGCTGTGTAGACGA
Suggested primer 2:AGTCCCTTCCAGTTGTCCCT