| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174916 |
| Chromosome: | chromosome 10 |
| Location: | 3124017 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441800 | (1 of 4) PF05066 - HB1, ASXL, restriction endonuclease HTH domain (HARE-HTH) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTCGAGAACAGGGGCTCGAGAACATGG |
| Internal bar code: | GGACCTGCAGACGGTGGGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 69 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTAGCCTCTGGGGTCTT |
| Suggested primer 2: | GCCAAAGAGTCCAAGCAGAC |