Insertion junction: LMJ.RY0402.174931_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.174931
Chromosome:chromosome_16
Location:5636021
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g679550 FAP277 Flagellar Associated Protein, protease inhibitor-like sense CDS/intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGGGGTTGGGGGTGGGGGAGCGGGGTTCG
Internal bar code:ATATAGTCAAGTCTCGTTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:676
LEAP-Seq percent confirming:99.661
LEAP-Seq n confirming:294
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTACAAACCGACCAGTGA
Suggested primer 2:AGAGAGAGGGCTGAAGGACC