Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.174931 |
Chromosome: | chromosome_16 |
Location: | 5636027 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g679550 | FAP277 | Flagellar Associated Protein, protease inhibitor-like | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTTTCCTGGCGAGTTCACCACGGGCGCCAA |
Internal bar code: | CGCAGCCGACGTGGCGTGAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 791 |
LEAP-Seq percent confirming: | 99.5966 |
LEAP-Seq n confirming: | 1975 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGTACAAACCGACCAGTGA |
Suggested primer 2: | AGAGAGAGGGCTGAAGGACC |