| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174962 |
| Chromosome: | chromosome 1 |
| Location: | 3773903 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024350 | (1 of 2) PTHR10766//PTHR10766:SF49 - TRANSMEMBRANE 9 SUPERFAMILY PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCTTCAAGGTGCGGTACTCATCAGGAAA |
| Internal bar code: | ATGGTCATGTGGAAAGAGGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 99.7599 |
| LEAP-Seq n confirming: | 1662 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCTGTCGTCGTTGTACT |
| Suggested primer 2: | CACTGTGAGTGCGGTAGGAA |