| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174990 |
| Chromosome: | chromosome 16 |
| Location: | 2107859 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657800 | CCD3,CCD2 | (1 of 2) K00464 - all-trans-8'-apo-beta-carotenal 15,15'-oxygenase (diox1); Carotenoid cleavage dioxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGGCCGTGTGACGCCGCACCGCCTGC |
| Internal bar code: | AACTTGATTGTCGCTGAATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 63 |
| LEAP-Seq percent confirming: | 99.4083 |
| LEAP-Seq n confirming: | 336 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAATCAAAGCGCAAAGA |
| Suggested primer 2: | TGTTCAGCAAGTGCTCCATC |