| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174992 |
| Chromosome: | chromosome 9 |
| Location: | 4758014 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397253 | (1 of 1) PTHR11748//PTHR11748:SF75 - D-LACTATE DEHYDROGENASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGGACTCGTGGGTCTCCAGTGTGCAAG |
| Internal bar code: | ATCCGGATTCGAGGTCCACGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 99.9389 |
| LEAP-Seq n confirming: | 3274 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | ATAGACGGGTTGTGAGGACG |