| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174993 |
| Chromosome: | chromosome 10 |
| Location: | 3417176 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444500 | (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACGGCTGCCTTGGCTGGGTATGGCTTC |
| Internal bar code: | CTATTGGCTATGCGCCTAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1260 |
| LEAP-Seq percent confirming: | 99.5188 |
| LEAP-Seq n confirming: | 1034 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCTTGCCTTGTTCAGTC |
| Suggested primer 2: | TATCGTGCTAATGCCTGTGC |