| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175007 |
| Chromosome: | chromosome 7 |
| Location: | 5680088 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352450 | SDR10 | Short-chain dehydrogenase/reductase; (1 of 3) 1.1.1.30 - 3-hydroxybutyrate dehydrogenase / D-beta-hydroxybutyrate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGCTGAACACGCTCCAACTGTGTTATT |
| Internal bar code: | CTACCCGACCATTCTAGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1043 |
| LEAP-Seq percent confirming: | 99.1337 |
| LEAP-Seq n confirming: | 801 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTGGTAAATGACGTTGT |
| Suggested primer 2: | TGAGGTAGACGGGGTACAGG |