| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.175029 |
| Chromosome: | chromosome 1 |
| Location: | 6249675 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044700 | GST4 | Glutathione S-transferase; (1 of 1) PTHR11260//PTHR11260:SF156 - GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACCTGTGCGCGACAATGGACACACACA |
| Internal bar code: | GGACTTCCAGACGACGGTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 169 |
| LEAP-Seq percent confirming: | 99.7136 |
| LEAP-Seq n confirming: | 3134 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGCATGGTAAGGGGGAG |
| Suggested primer 2: | GTAGTTGGTGTTGGGGATGG |