| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175033 |
| Chromosome: | chromosome 16 |
| Location: | 2629088 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661900 | HEL61 | (1 of 3) PTHR24031//PTHR24031:SF232 - RNA HELICASE // ATP-DEPENDENT RNA HELICASE TDRD12-RELATED; DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGCAGGTGTTGCTGTTGTTGCTGCTG |
| Internal bar code: | GTCGGCCGCGGCAGCGATGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 377 |
| LEAP-Seq percent confirming: | 84.7795 |
| LEAP-Seq n confirming: | 1788 |
| LEAP-Seq n nonconfirming: | 321 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCAATGCCTGTGCAAGAA |
| Suggested primer 2: | CGTCAGGTAAGGCTGAGAGG |