| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.175058 |
| Chromosome: | chromosome 17 |
| Location: | 4439803 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732050 | AGE3 | DNA repair glycosylase; (1 of 1) K10773 - endonuclease III (NTH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACGCACCTGGTCAGCCGCGCCCCGCAG |
| Internal bar code: | TAATCCTGCAGTTGTCATAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1110 |
| LEAP-Seq percent confirming: | 52.8736 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCAGTCGACACACACAT |
| Suggested primer 2: | CTTCCGTTCCTATCGTCAGC |