Insertion junction: LMJ.RY0402.175076_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.175076
Chromosome:chromosome 8
Location:853596
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g361150 GT90F11,GT90-11 GT90 family protein 11; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTTCCCACCTCTTTGTCACGCGCTGGCA
Internal bar code:CAGTAAACTTCTCCCAGTCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:984
LEAP-Seq percent confirming:99.7872
LEAP-Seq n confirming:938
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGGGTAGTTTATTCCCAT
Suggested primer 2:CCCTTTCAGCATTTTTCCAA