Insertion junction: LMJ.RY0402.175084_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.175084
Chromosome:chromosome 1
Location:4647294
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g031951 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGCCAAGATCCGGTGAGTGACGATTTCG
Internal bar code:CGAACCCTCGCAACGCTGGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:275
LEAP-Seq percent confirming:99.6094
LEAP-Seq n confirming:1275
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AACACAGTCACAGCCACAGC
Suggested primer 2:CGTCATCATTTTCATCCACG