| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.175085 |
| Chromosome: | chromosome 13 |
| Location: | 4198995 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592300 | (1 of 1) 1.3.1.91 - tRNA-dihydrouridine(20) synthase (NAD(P)(+)) / tRNA-dihydrouridine synthase 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGAAGGTCTACCAAGCGAGCCTCATT |
| Internal bar code: | GGGATTCAGGAGGTAGGTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 826 |
| LEAP-Seq percent confirming: | 99.1189 |
| LEAP-Seq n confirming: | 225 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACAGCACCAGACGCAAAA |
| Suggested primer 2: | CTGCAACATGCGACCTTAGA |