| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175089 |
| Chromosome: | chromosome 15 |
| Location: | 877958 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639503 | GEX173 | (1 of 7) PTHR23257//PTHR23257:SF474 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGCCCCAAGCCCCCCAAAACGTCAACG |
| Internal bar code: | CGGCTACGTGTAGGCGGAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 88.9336 |
| LEAP-Seq n confirming: | 442 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGCTAATAGCGCGTTCC |
| Suggested primer 2: | TCATTAGCGTCATCAGCAGC |