Insertion junction: LMJ.RY0402.175091_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.175091
Chromosome:chromosome 11
Location:1419079
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467725 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGGTGAGGTGTTTTGGCGCTTGGGGCC
Internal bar code:ACTTGGCCGGCGCGGCAGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:148
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAACAACATCACGAACGGTG
Suggested primer 2:TACGGTAAATGTTCCTCGCC