| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175094 |
| Chromosome: | chromosome 8 |
| Location: | 3349643 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374150 | (1 of 11) IPR011146 - HIT-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCACCGGCTGTAGCAGCAGCATCACCGG |
| Internal bar code: | GCGCAAGTAAAAGGAGGGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 94.8454 |
| LEAP-Seq n confirming: | 1288 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTTTGACACCTACCACT |
| Suggested primer 2: | CTTGCTTGTGTTTGTGCTGG |