| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175098 |
| Chromosome: | chromosome 9 |
| Location: | 846025 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401750 | (1 of 3) PTHR10696 - GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED; Similar to conserved hypothetical protein in bacteria | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCACGAGGTCTGCCAGAGCACGTCGTA |
| Internal bar code: | GAGTAGGTACAAGCCAGCAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 24.7794 |
| LEAP-Seq n confirming: | 2359 |
| LEAP-Seq n nonconfirming: | 7161 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGGTGCGAGATTATGA |
| Suggested primer 2: | ATCAAGACGGCTACCGACAC |