| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175126 |
| Chromosome: | chromosome 3 |
| Location: | 8135761 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200700 | FAP166 | (1 of 79) IPR011992 - EF-hand domain pair; Flagellar Associated Protein 166 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGAGCCCCCACATGCCACTACCGCATC |
| Internal bar code: | TAGCTCACCGCGGATGGCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 99.7847 |
| LEAP-Seq n confirming: | 3707 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGCCTTTGTGTCTATGC |
| Suggested primer 2: | GCAAGGCTACGAGGTCACTC |