Insertion junction: LMJ.RY0402.175127_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.175127
Chromosome:chromosome 12
Location:3669466
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g514400 (1 of 1) PTHR12802//PTHR12802:SF60 - SWI/SNF COMPLEX-RELATED // PROTEIN REVEILLE 1-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTTGCCCAACCGCGCCTACAAAATTAAA
Internal bar code:GTGCACCCCGCCTACTTTCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:966
LEAP-Seq percent confirming:99.6814
LEAP-Seq n confirming:3754
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTCTCACTACCTTCTCC
Suggested primer 2:AAATTACAAGGCGTTGGCAC