| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175130 |
| Chromosome: | chromosome 17 |
| Location: | 2501976 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716101 | POLL1 | (1 of 1) PF10391 - Fingers domain of DNA polymerase lambda (DNA_pol_lambd_f); DNA polymerase lambda | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCGGGAGCCAGTACGGCACGTGGCCTT |
| Internal bar code: | GGGCGCAGATCTTCAAGTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 90.9457 |
| LEAP-Seq n confirming: | 8588 |
| LEAP-Seq n nonconfirming: | 855 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGGTTGCGTACTGTTTAT |
| Suggested primer 2: | CTTAGCACGGTGACCCAGTT |