| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175141 |
| Chromosome: | chromosome 5 |
| Location: | 2748434 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236878 | NAT15 | (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGCGCATCAGCGCATGGGTCCTGTTCC |
| Internal bar code: | GCGCATGCCCTCTTAATTCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 495 |
| LEAP-Seq percent confirming: | 95.451 |
| LEAP-Seq n confirming: | 1217 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGAAGATGCAACAGAGA |
| Suggested primer 2: | GCACGTTTTCATGCAAGCTA |