| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175152 |
| Chromosome: | chromosome 8 |
| Location: | 846460 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361050 | CNX3 | (1 of 2) 4.1.99.18 - Cyclic pyranopterin phosphate synthase / Molybdenum cofactor biosynthesis protein 1; Molybdenum cofactor synthesis-step 1 protein | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGAGAGGGGGGAGTCTGGGGAGGTGGC |
| Internal bar code: | CATGTTGCGGGTCCGTTTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCAACCCCGATTTATTT |
| Suggested primer 2: | GGTGTATCTGGGTGACGCTT |