| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175164 |
| Chromosome: | chromosome 12 |
| Location: | 6313209 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTGAGGGGTGGGGATGATGGTGATGGT |
| Internal bar code: | GTCTTCGCACATAGGACAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 98.3369 |
| LEAP-Seq n confirming: | 3252 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTTGGTGACGCTGGTA |
| Suggested primer 2: | GCCGTGTCATATACCTGCCT |