| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175170 |
| Chromosome: | chromosome 10 |
| Location: | 5362272 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458216 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGGGGGGGATGGTAACTCACTCAAGA |
| Internal bar code: | GGAATGGCAGCGCTCGCTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 99.6678 |
| LEAP-Seq n confirming: | 300 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCAAAACCCACCACAGAC |
| Suggested primer 2: | TGCCGTCAAACAGCTATGAG |