Insertion junction: LMJ.RY0402.175172_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.175172
Chromosome:chromosome 7
Location:3657768
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g337300 DSK2,DYRK2,DYRKP1,DYRKP,STD1 Dual-Specificity Tyrosine-Regulated Protein Kinase involved in starch degradation; (1 of 2) PTHR24058//PTHR24058:SF23 - DUAL SPECIFICITY PROTEIN KINASE // SERINE/THREONINE KINASE-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTACACGCTTGCGTACGACGGCCCTGAA
Internal bar code:CGGTCGGTGATACATCGCTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:92
LEAP-Seq percent confirming:99.5122
LEAP-Seq n confirming:204
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTACAGGGCAGCTAGGCAAG
Suggested primer 2:CAAGCAAGGAGTCAGGAAGG