| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.175197 |
| Chromosome: | chromosome_1 |
| Location: | 3545681 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g022750 | FAP159 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCCTCCTGCATGCGCTGCACCCAGTCGGG |
| Internal bar code: | ATGTCCGACCCCTCAACACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 99.4638 |
| LEAP-Seq n confirming: | 371 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTCCACCTGCTCTGAC |
| Suggested primer 2: | GGTCTGTACGACACTGGTGG |