| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.175201 |
| Chromosome: | chromosome_1 |
| Location: | 1749643 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009400 | NSS2 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCACCCAAAGGAAAGGTTTGACACAGACC |
| Internal bar code: | AGTAACGGGGCACGGATGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 968 |
| LEAP-Seq percent confirming: | 94.5899 |
| LEAP-Seq n confirming: | 542 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAAAAACTACGCCCCGAC |
| Suggested primer 2: | CCACCACCTGCACTACCTTT |