| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.175208 |
| Chromosome: | chromosome 4 |
| Location: | 1599933 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213250 | (1 of 2) K01256 - aminopeptidase N (pepN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCCGACGCTCACGTCGCCAGCCCAAAC |
| Internal bar code: | ATGCGGGAGGGTCAGTTCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 991 |
| LEAP-Seq percent confirming: | 88.8315 |
| LEAP-Seq n confirming: | 12559 |
| LEAP-Seq n nonconfirming: | 1579 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCTGTTTCCTTGCTGTC |
| Suggested primer 2: | ACCTTCCACCGAACACTCAC |