| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.175211 |
| Chromosome: | chromosome 6 |
| Location: | 2194340 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266000 | STK1 | Serine/threonine protein kinase; (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTATGACCCCGGATACGGTAATGTCCA |
| Internal bar code: | TAACATATTGGGTTCTCCTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1085 |
| LEAP-Seq percent confirming: | 99.4224 |
| LEAP-Seq n confirming: | 11533 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGGTGGCTGAACGAGTA |
| Suggested primer 2: | CTCAAGCCCCTACACAGCTC |